LEGEND MAX(TM) Total TGF-beta1 ELISA Kit 436707 from BioLegend

Product Specs
ItemLEGEND MAX(TM) Total TGF-beta1 ELISA Kit
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number436707
ReactivityHuman, Mouse, Rat, Non-Human Primate, Pig, Cow, Rabbit
ApplicationsELISA
MethodELISA
Quantity1 Pre-coated Plate
NCBI Full Gene Nametransforming growth factor, beta 1
NCBI Gene AliasesTGF-beta1, TGFbeta1, Tgfb, Tgfb-1
Detection TargetTGF-beta1

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Engineering human stem cell-derived islets to evade immune rejection and promote localized immune toleranceCell Reports MedicineJanuary 17, 2023Dario Gerace, Quan Zhou, Jennifer Hyoje-Ryu Kenty, Adrian Veres, Elad Sintov, Xi Wang, Kyle R. Boulanger, Hongfei Li, Douglas A. MeltonIL-2, TGF-β and IL-10 quantified using Legend Max ELISAs (IL-2, Biolegend, 431807; TGF-β, Biolegend, 436707; IL-10, Biolegend, 430607) according to the manufacturer’s instructions. For cytotoxicity assays, WT

(2) Microglia regulate central nervous system myelin growth and integrityNatureJanuary 1, 2023McNamara NB, Munro DAD, Bestard-Cuche N, Uyeda A, Bogie JFJ, Hoffmann A, Holloway RK, Molina-Gonzalez I, Askew KE, Mitchell S, et al.callosum lysate samples were activated and TGFβ1 protein levels were measured by ELISA (BioLegend, 436707) according to the manufacturer’s instructions for serum and plasma samples at a final dilution of 1:10.

(3) Recruited monocytes/macrophages drive pulmonary neutrophilic inflammation and irreversible lung tissue remodeling in cystic fibrosisCell reportsDecember 13, 2022Öz HH, Cheng EC, Di Pietro C, Tebaldi T, Biancon G, Zeiss C, Zhang PX, Huang PH, Esquibies SS, Britto CJ, et al.assessed by ELISA following manufacturer’s instruction (active free form: 437707 Biolegend; total levels: 436707 Biolegend; CXCL2: DY452-05 R&D Systems). QUANTIFICATION AND STATISTICAL ANALYSIS All data presented

(4) Serum of Post-COVID-19 Syndrome Patients with or without ME/CFS Differentially Affects Endothelial Cell Function In VitroCellsAugust 2, 2022Lavinia Flaskamp, Constanze Roubal, Steven Uddin, Franziska Sotzny, Claudia Kedor, Sandra Bauer, Carmen Scheibenbogen, Martina Seifert(TGF-β1) and VEGF in the serum samples were assessed using the LEGEND MAXTM Total TGF-β1 ELISA kit (#436707, BioLegend, San Diego, CA, USA) and the VEGF ELISA kit (#DVE00, R&D Systems, Minneapolis, MN, USA),

(5) Plasmodium berghei-Released Factor, PbTIP, Modulates the Host Innate Immune ResponsesFrontiers in ImmunologyDecember 7, 2021Inderjeet Kalia, Rajesh Anand, Afshana Quadiri, Shreya Bhattacharya, Bijayalaxmi Sahoo, Agam Prasad Singhmedium was used for ELISA following the manufacturer’s protocol (BioLegend cat. nos. 432604, 431604, 436707, and 431411; Becton Dickinson cat nos. 560478 and 555138). Parasite Culture for Schizonts C57BL/6J

(6) Systemic Delivery of Anti-Integrin αL Antibodies Reduces Early Macrophage Recruitment, Inflammation, and Scar Formation in Murine Burn WoundsAdvances in wound careDecember 1, 2020Strudwick XL, Adams DH, Pyne NT, Samuel MS, Murray RZ, Cowin AJ.

(7) Hepatitis B envelope antigen increases Tregs by converting CD4+CD25^- T cells into CD4⁺CD25⁺Foxp3⁺ TregsExperimental and therapeutic medicineOctober 1, 2020Tang R, Lei Z, Wang X, Qi Q, He J, Liu D, Wang X, Chen X, Zhu J, Li Y, et al.in the supernatants of spleen cells were determined with commercial ELISA kits (cat. nos. 437707 and 436707; BioLegend, Inc.) according to the manufacturer's instructions. The cytokine concentration in each supernatant

(8) Microbiota Composition of Breast Milk from Women of Different Ethnicity from the Manawatu-Wanganui Region of New ZealandNutrientsJune 11, 2020Butts CA, Paturi G, Blatchford P, Bentley-Hewitt KL, Hedderley DI, Martell S, Dinnan H, Eady SL, Wallace AJ, Glyn-Jones S, et al.was determined using the LEGEND MAX™ Total TGFβ1 ELISA kit (Biolegend, San Diego, CA, USA, Cat No: 436707), with a minimal detectable concentration of 3.5 pg/mL, an intra-assay precision of <4% and an inter-assay precision

(9) Myosin II Reactivation and Cytoskeletal Remodeling as a Hallmark and a Vulnerability in Melanoma Therapy ResistanceCancer cellJanuary 13, 2020Orgaz JL, Crosas-Molist E, Sadok A, Perdrix-Rosell A, Maiques O, Rodriguez-Hernandez I, Monger J, Mele S, Georgouli M, Bridgeman V, et al.TGF-β1 levels were detected by ELISA using Total TGF-β1 Legend Max™ ELISA Kit with Pre-coated plate (#436707, Biolegend) on neat samples diluted 1/5 following the manufacturer’s instructions. Phospho-proteomics Preparation

(10) Human Cytomegalovirus miRNAs Regulate TGF-β to Mediate Myelosuppression while Maintaining Viral Latency in CD34⁺ Hematopoietic Progenitor CellsCell host & microbeJanuary 8, 2020Hancock MH, Crawford LB, Pham AH, Mitchell J, Struthers HM, Yurochko AD, Caposio P, Nelson JA.Magnetic 30-plex panel,ThermoFisher,Cat# LHC6003MCytokine LegendMAX total TGF-β1 ELISA,Biolegend,Cat# 436707 SMAD3 Taqman Assay,ThermoFisher,Hs00969210_m1 SERPINE Taqman Assay,ThermoFisher,Hs00167155_m1 18S

(11) Semaphorin 4D in human head and neck cancer tissue and peripheral blood: A dense fibrotic peri-tumoral stromal phenotypeOncotargetFebruary 16, 2018Derakhshandeh R, Sanadhya S, Lee Han K, Chen H, Goloubeva O, Webb TJ, Younis RH.tumor cells, Human ELISA TGF-β1 total kit was used following manufacturer recommendations (catalog no.436707) (BioLegend, San Diego, CA). Conditioned medium was run in triplicates and plates were read using BioTek

(12) Enhanced cytomegalovirus infection in human trabecular meshwork cells and its implication in glaucoma pathogenesisScientific reportsFebruary 27, 2017Choi JA, Kim JE, Noh SJ, Kyoung Kim E, Park CK, Paik SY.

(13) Electronegative low density lipoprotein induces renal apoptosis and fibrosis: STRA6 signaling involvedJournal of lipid researchAugust 1, 2016Chen CH, Ke LY, Chan HC, Lee AS, Lin KD, Chu CS, Lee MY, Hsiao PJ, Hsu C, Chen CH, et al.

(14) Cartilage-Specific Knockout of the Mechanosensory Ion Channel TRPV4 Decreases Age-Related OsteoarthritisScientific reportsJuly 8, 2016O'Conor CJ, Ramalingam S, Zelenski NA, Benefield HC, Rigo I, Little D, Wu CL, Chen D, Liedtke W, McNulty AL, et al.at sacrifice for biomarker analysis57. Total TGF-β1 was measured using Sandwich ELISA (Legend Max #436707, BioLegend, San Diego, CA, USA). Samples were run undiluted as recommended by the manufacturer. For

(15) Precision-Cut Kidney Slices as a Tool to Understand the Dynamics of Extracellular Matrix Remodeling in Renal FibrosisBiomarker InsightsMay 22, 2016Federica Genovese, Zsolt S. Kàrpàti, Signe H. Nielsen, Morten A. Karsdalgrowth factor (TGF)-β was assessed using a commercial ELISA developed by BioLegend (Cat. 437707 and Cat. 436707). Statistical analysis All results are presented as mean ± standard error of the mean (SEM). The statistical

(16) Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor CellsJournal of immunology (Baltimore, Md. : 1950)February 1, 2016Younis RH, Han KL, Webb TJ.(catalog no. E2311; Promega). ELISA Human ELISA MAX IFN-g (catalog no. 430103), TGF-b1 total (catalog no. 436707), IL-10 (catalog no. 430601), and IL-4 (catalog no. 430301) were obtained from BioLegend. Samples were

(17) Insulin-like growth factor-1 induces regulatory T cell-mediated suppression of allergic contact dermatitis in miceDisease models & mechanismsAugust 1, 2014Johannesson B, Sattler S, Semenova E, Pastore S, Kennedy-Lydon TM, Sampson RD, Schneider MD, Rosenthal N, Bilbao D.

(18) Distinct antifibrogenic effects of erlotinib, sunitinib and sorafenib on rat pancreatic stellate cellsWorld journal of gastroenterologyJune 28, 2014Elsner A, Lange F, Fitzner B, Heuschkel M, Krause BJ, Jaster R.clearing the lysates by centrifugation, TGF-β1 concentrations were measured using a commercial ELISA (#436707, BioLegend, San Diego, CA, United States) according to the manufacturer’s instructions. Detection of

(19) Targeting pro-inflammatory cytokines following joint injury: acute intra-articular inhibition of interleukin-1 following knee injury prevents post-traumatic arthritisArthritis Research & TherapyJune 25, 2014Bridgette D Furman, Daniel S Mangiapani, Evan Zeitler, Karsyn N Bailey, Phillip H Horne, Janet L Huebner, Virginia B Kraus, Farshid Guilak, Steven A Olsontransforming growth factor (TGF)-β1 (BioLegend, #437707, San Diego, CA, USA) and total TGF-β1 (BioLegend, #436707). Samples were run undiluted for FA TGF-β1 and diluted 1,000-fold for total TGF-β1 as recommended by

(20) The Epstein-Barr virus causes epithelial-mesenchymal transition in human corneal epithelial cells via Syk/src and Akt/Erk signaling pathwaysInvestigative ophthalmology & visual scienceMarch 20, 2014Park GB, Kim D, Kim YS, Kim S, Lee HK, Yang JW, Hur DY.

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LEGEND MAX(TM) Total TGF-beta1 ELISA Kit from BioLegend

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash

LEGEND MAX(TM) Total TGF-beta1 ELISA Kit from BioLegend

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